| Solution Information | help | |
| Enzyme: | cAMP-dependent protein kinase type I-alpha regulatory subunit | |
| inhibitor: | BDBM66980 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this biochemical assay is to determine dose response curves of compounds identified as active in a set of previous experiments entitled, "Fluorescence polarization-based biochemical primary high throughput screening assay to identify activators of the Protein Kinase A-R1A (PKA-R1A) complex" (AID 504707) and that confirmed activity in a set of experiments entitled, "Fluorescence polarization-based biochemical high throughput confirmation assay for activators of the Protein Kinase A-R1A (PKA-R1A) complex" (AID 504800) and that were inactive in a set of experiments entitled, "Counterscreen for activators of the Protein Kinase A-R1A (PKA-R1A) complex: fluorescence polarization-based biochemical high throughput screening assay to identify fluorescence polarization assay artifacts" (AID 504799). This fluorescent polarization-based assay monitors activation of PKA holoenzyme (10) to determine the ability of compounds to induce the dissociation of the PKA-R1A | |
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